PHOTOSENSITIZATION BY PORPHYRINS DELIVERED TO L CELL FIBROBLASTS BY HUMAN SERUM LOW DENSITY LIPOPROTEINS. A MICROSPECTROFLUOROMETRIC STUDY
Identifieur interne : 000459 ( France/Analysis ); précédent : 000458; suivant : 000460PHOTOSENSITIZATION BY PORPHYRINS DELIVERED TO L CELL FIBROBLASTS BY HUMAN SERUM LOW DENSITY LIPOPROTEINS. A MICROSPECTROFLUOROMETRIC STUDY
Auteurs : P. Morliere [France] ; E. Kohen [États-Unis] ; J. P. Reyftmann [France] ; R. Santus [France] ; C. Kohen [États-Unis] ; J. C. Maziere [France] ; S. Goldstein [France] ; W. F. Mangel [États-Unis] ; L. Dubertret [France]Source :
- Photochemistry and Photobiology [ 0031-8655 ] ; 1987-08.
Abstract
Human serum LDL were used as vehicles to deliver protoporphyrin and hematoporphyrin dimer to L cell mouse fibroblasts. Topographic analysis by microspectrofluorometry on single living cells shows that after digestion of LDL, protoporphyrin is localized in cytoplasmic areas. Protoporphyrin and hematoporphyrin dimer are readily bleached by 420 ± 60 nm radiations at the high fluence rate used. Complex bleaching kinetics are observed. Spectral studies using the same technique demonstrate that an intense fluorescent emission (λmax= 450 nm) is produced immediately after the onset of irradiation with 365 ± 2 nm or 420 ± 60 nm radiations using LDL loaded with protoporphyrin or Photofrin II. These fluorescent products have been previously identified as lipofuscin‐like pigments formed by reaction of lipid photoperoxides with amino groups. The permeation of lysosomal membranes is also induced after delivery of the porphyrins by LDL. This permeation can be strongly inhibited not only by the lysosomal inhibitors chloroquine and monensin but also by a‐tocopherol. On the other hand, neither α‐tocopherol nor chloroquine or monensin inhibit the lipofuscin‐like pigment formation.
Url:
DOI: 10.1111/j.1751-1097.1987.tb04755.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000684
- to stream Istex, to step Curation: 000684
- to stream Istex, to step Checkpoint: 002103
- to stream Main, to step Merge: 003416
- to stream Main, to step Curation: 003344
- to stream Main, to step Exploration: 003344
- to stream France, to step Extraction: 000459
Links to Exploration step
ISTEX:323BACF2A21611B15C4214FFFA4105778320E885Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">PHOTOSENSITIZATION BY PORPHYRINS DELIVERED TO L CELL FIBROBLASTS BY HUMAN SERUM LOW DENSITY LIPOPROTEINS. A MICROSPECTROFLUOROMETRIC STUDY</title><author><name sortKey="Morliere, P" sort="Morliere, P" uniqKey="Morliere P" first="P." last="Morliere">P. Morliere</name></author><author><name sortKey="Kohen, E" sort="Kohen, E" uniqKey="Kohen E" first="E." last="Kohen">E. Kohen</name></author><author><name sortKey="Reyftmann, J P" sort="Reyftmann, J P" uniqKey="Reyftmann J" first="J. P." last="Reyftmann">J. P. Reyftmann</name></author><author><name sortKey="Santus, R" sort="Santus, R" uniqKey="Santus R" first="R." last="Santus">R. Santus</name></author><author><name sortKey="Kohen, C" sort="Kohen, C" uniqKey="Kohen C" first="C." last="Kohen">C. Kohen</name></author><author><name sortKey="Maziere, J C" sort="Maziere, J C" uniqKey="Maziere J" first="J. C." last="Maziere">J. C. Maziere</name></author><author><name sortKey="Goldstein, S" sort="Goldstein, S" uniqKey="Goldstein S" first="S." last="Goldstein">S. Goldstein</name></author><author><name sortKey="Mangel, W F" sort="Mangel, W F" uniqKey="Mangel W" first="W. F." last="Mangel">W. F. Mangel</name></author><author><name sortKey="Dubertret, L" sort="Dubertret, L" uniqKey="Dubertret L" first="L." last="Dubertret">L. Dubertret</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:323BACF2A21611B15C4214FFFA4105778320E885</idno><date when="1987" year="1987">1987</date><idno type="doi">10.1111/j.1751-1097.1987.tb04755.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-G3RKNQ4W-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000684</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000684</idno><idno type="wicri:Area/Istex/Curation">000684</idno><idno type="wicri:Area/Istex/Checkpoint">002103</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002103</idno><idno type="wicri:doubleKey">0031-8655:1987:Morliere P:photosensitization:by:porphyrins</idno><idno type="wicri:Area/Main/Merge">003416</idno><idno type="wicri:Area/Main/Curation">003344</idno><idno type="wicri:Area/Main/Exploration">003344</idno><idno type="wicri:Area/France/Extraction">000459</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">PHOTOSENSITIZATION BY PORPHYRINS DELIVERED TO L CELL FIBROBLASTS BY HUMAN SERUM LOW DENSITY LIPOPROTEINS. A MICROSPECTROFLUOROMETRIC STUDY</title><author><name sortKey="Morliere, P" sort="Morliere, P" uniqKey="Morliere P" first="P." last="Morliere">P. Morliere</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Hôpital H. Mondor, Laboratoire de Recherche Dermatologique, 94010, Creteil</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Créteil</settlement></placeName></affiliation></author><author><name sortKey="Kohen, E" sort="Kohen, E" uniqKey="Kohen E" first="E." last="Kohen">E. Kohen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Miami, Department of Biology, POB 249 118 Coral Gables, FL. 33124</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Reyftmann, J P" sort="Reyftmann, J P" uniqKey="Reyftmann J" first="J. P." last="Reyftmann">J. P. Reyftmann</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physico‐Chimie de ľAdaptation Biologique, UA 481 du CNRS, 43, rue Cuvier, 75231, Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Santus, R" sort="Santus, R" uniqKey="Santus R" first="R." last="Santus">R. Santus</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physico‐Chimie de ľAdaptation Biologique, UA 481 du CNRS, 43, rue Cuvier, 75231, Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Kohen, C" sort="Kohen, C" uniqKey="Kohen C" first="C." last="Kohen">C. Kohen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Miami, Department of Biology, POB 249 118 Coral Gables, FL. 33124</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Maziere, J C" sort="Maziere, J C" uniqKey="Maziere J" first="J. C." last="Maziere">J. C. Maziere</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biochimie, UA 571 du CNRS, Faculté de Médecine St Antoine, 27, rue Chaligny, 75012, Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Goldstein, S" sort="Goldstein, S" uniqKey="Goldstein S" first="S." last="Goldstein">S. Goldstein</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biochimie, UA 571 du CNRS, Faculté de Médecine St Antoine, 27, rue Chaligny, 75012, Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Mangel, W F" sort="Mangel, W F" uniqKey="Mangel W" first="W. F." last="Mangel">W. F. Mangel</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Brookhaven National Laboratory, Biology Department, Upton N.Y., 11973</wicri:regionArea><wicri:noRegion>11973</wicri:noRegion></affiliation></author><author><name sortKey="Dubertret, L" sort="Dubertret, L" uniqKey="Dubertret L" first="L." last="Dubertret">L. Dubertret</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Hôpital H. Mondor, Laboratoire de Recherche Dermatologique, 94010, Creteil</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Créteil</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Photochemistry and Photobiology</title><title level="j" type="alt">PHOTOCHEMISTRY PHOTOBIOLOGY</title><idno type="ISSN">0031-8655</idno><idno type="eISSN">1751-1097</idno><imprint><biblScope unit="vol">46</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="183">183</biblScope><biblScope unit="page" to="191">191</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1987-08">1987-08</date></imprint><idno type="ISSN">0031-8655</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0031-8655</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human serum LDL were used as vehicles to deliver protoporphyrin and hematoporphyrin dimer to L cell mouse fibroblasts. Topographic analysis by microspectrofluorometry on single living cells shows that after digestion of LDL, protoporphyrin is localized in cytoplasmic areas. Protoporphyrin and hematoporphyrin dimer are readily bleached by 420 ± 60 nm radiations at the high fluence rate used. Complex bleaching kinetics are observed. Spectral studies using the same technique demonstrate that an intense fluorescent emission (λmax= 450 nm) is produced immediately after the onset of irradiation with 365 ± 2 nm or 420 ± 60 nm radiations using LDL loaded with protoporphyrin or Photofrin II. These fluorescent products have been previously identified as lipofuscin‐like pigments formed by reaction of lipid photoperoxides with amino groups. The permeation of lysosomal membranes is also induced after delivery of the porphyrins by LDL. This permeation can be strongly inhibited not only by the lysosomal inhibitors chloroquine and monensin but also by a‐tocopherol. On the other hand, neither α‐tocopherol nor chloroquine or monensin inhibit the lipofuscin‐like pigment formation.</div></front></TEI><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Floride</li><li>Île-de-France</li></region><settlement><li>Créteil</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Morliere, P" sort="Morliere, P" uniqKey="Morliere P" first="P." last="Morliere">P. Morliere</name></region><name sortKey="Dubertret, L" sort="Dubertret, L" uniqKey="Dubertret L" first="L." last="Dubertret">L. Dubertret</name><name sortKey="Goldstein, S" sort="Goldstein, S" uniqKey="Goldstein S" first="S." last="Goldstein">S. Goldstein</name><name sortKey="Maziere, J C" sort="Maziere, J C" uniqKey="Maziere J" first="J. C." last="Maziere">J. C. Maziere</name><name sortKey="Reyftmann, J P" sort="Reyftmann, J P" uniqKey="Reyftmann J" first="J. P." last="Reyftmann">J. P. Reyftmann</name><name sortKey="Santus, R" sort="Santus, R" uniqKey="Santus R" first="R." last="Santus">R. Santus</name></country><country name="États-Unis"><region name="Floride"><name sortKey="Kohen, E" sort="Kohen, E" uniqKey="Kohen E" first="E." last="Kohen">E. Kohen</name></region><name sortKey="Kohen, C" sort="Kohen, C" uniqKey="Kohen C" first="C." last="Kohen">C. Kohen</name><name sortKey="Mangel, W F" sort="Mangel, W F" uniqKey="Mangel W" first="W. F." last="Mangel">W. F. Mangel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000459 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000459 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:323BACF2A21611B15C4214FFFA4105778320E885
|texte= PHOTOSENSITIZATION BY PORPHYRINS DELIVERED TO L CELL FIBROBLASTS BY HUMAN SERUM LOW DENSITY LIPOPROTEINS. A MICROSPECTROFLUOROMETRIC STUDY
}}
| This area was generated with Dilib version V0.6.33. |  |